STUDIES ON POLYNUCLEOTIDES SYNTHESIZED BY POLYNUCLEOTIDE PHOSPHORYLASE
نویسندگان
چکیده
منابع مشابه
Studies on Polynucleotides Synthesized by Polynucleotide Phosphorylase
The preparation of synthetic ribonucleic acid in which one of the nucleotide species is labeled with P32, makes possible studies on the distribution of the labeled nucleotide in the polynucleotide chain. Fig. 1 presents the structure of a polynucleotide in which labeled adenylic acid is randomly distributed. Hydrolysis of such a polymer with snake venom phosphodiesterase (Fig. 1A) will yield a ...
متن کاملStudies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction occurs when the deoxynucleoside at a 5'-end carries a phosphate group and the complementary deoxynucleo...
متن کاملPolynucleotide phosphorylase of Micrococcus lysodeiktpcus. III. The apparent arsenolysis of nucleoside diphosphates by polynucleotide phosphorylase.
The enzyme, polynucleotide phosphorylase, catalyzes the polymerization of nucleoside diphosphates to polyribonucleotides with the formation of inorganic orthophosphate (1, 2). The reaction is readily reversible, and the phosphorolysis of polyribonucleotides has been studied extensively (3-6). Several recent reviews (7-9) afford extensive summaries of the literature. In the accompanying paper (l...
متن کاملPhosphorolysis of oligoribonucleotides by polynucleotide phosphorylase.
This reversible reaction has been demonstrated With enzyme preparations from Azotobacter trinelundii (1, 2) and Escherichia coli (3). In the forward direction the enzyme catalyzes the formation of polyribonucleotides similar in structural details to natural RNA1 (5-8). In the reverse reaction polyribonucleotides are phosphorolyzed to yield nucleoside diphosphates. The phosphorolysis of syntheti...
متن کاملThe dependence of reactions catalyzed by polynucleotide phosphorylase on oligonucleotides.
summarize our knowledge of the mechanism, specificity, and properties of enzyme preparations from several sources, and the purification procedures that have been reported. Previous studies by Singer, Heppel, and Hilmoe (3, 4) led to the suggestion that polynucleotide phosphorylase may be unable to catalyze the initiation of new polyribonucleotide chains, but rather that it acts by the elongatio...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)63675-6